Molecular Biology

Overview
The purpose of the Molecular Biology laboratory is to allow students to extract a DNA sample from any of the available species, amplify a segment of the D...
Wed, 14 Dec, 2022 at 12:11 PM
The Simulation
The overall goal of the Molecular Biology laboratory is to give students the opportunity to produce DNA sequences of amplified segments and genes and then...
Wed, 14 Dec, 2022 at 12:11 PM
The Laboratory
Once the student launches the Molecular Biology laboratory, the student is placed in front of a lab bench containing a large assortment of experimental equi...
Wed, 14 Dec, 2022 at 12:11 PM
Species Menu
At the core of the virtual biology environment is the Species Menu, which allows students to select a species and perform experiments on that species. Not a...
Wed, 14 Dec, 2022 at 12:12 PM
Controlling Time
When the molecular simulation is first started, the laboratory clock shows the current time of day in a 24-hour format. Many of the experiments in the labor...
Wed, 14 Dec, 2022 at 12:12 PM
Preparing Samples
Samples are prepared for PCR experiments by adding DNA from a species, Taq polymerase, either tagged or untagged nucleotides, and the correct primers requ...
Wed, 14 Dec, 2022 at 12:12 PM
Selecting Primers
 The Setup menu contains the Primer section, a list of available primers that can be added to the sample tubes. The primer list contains all primers that ar...
Wed, 14 Dec, 2022 at 12:12 PM
Running the PCR
The PCR machine takes up to 8 tubes of DNA samples and runs them through a three step temperature and hold profile for a specified number of cycles. If...
Wed, 14 Dec, 2022 at 12:12 PM
Gel Electrophoresis
In the gel electrophoresis experiment, amplified product is pipetted into one of 8 different wells in the gel and a voltage is applied across the gel bed to...
Wed, 14 Dec, 2022 at 12:12 PM
Using the Sequencer
Sequencers are used to determine the DNA sequence of the amplified product. Actual DNA sequencers are sophisticated pieces of equipment and require a unique...
Wed, 14 Dec, 2022 at 12:12 PM